Caspase 8 antibody (AA 25-175)

Catalog No. ABIN3201520

Product Details anti-Caspase 8 Antibody

Target: Caspase 8 (CASP8)

Binding Specificity: AA 25-175

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Monoclonal Antibody to Caspase 8 (CASP8)

Sequence: MGHHHHHHSG SEF-LSRDHI PYRKQEPIKD ALMLFQRLQE KRMLEENNLS FLKELLFRVN RLDLLLNYLY TSEEEMKREL QIPGRAQISA YRILLFQISE DVNKVELKDF KFFLSQEIAK CKLDDDMTLL DIFVEMEKRT ILGEENLDTL KRICEQVNKS LLKKI

Specificity: The antibody is a mouse monoclonal antibody raised against CASP8. It has been selected for its ability to recognize CASP8 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Caspase 8 (CASP8) corresdonding to Leu25~Ile175 (Accession # Q2LGB8)

Clone: 4#

Isotype: IgG2a kappa

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:500-2000 Immunohistochemistry: 5-20 μg/mL

1:50-200 Immunocytochemistry: 5-20 μg/mL

1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Caspase 8

Target: Caspase 8 (CASP8)

Alternative Name: Caspase 8 (CASP8 Products)

Synonyms: casp8-A antibody, xCaspase-8 antibody, casp8 antibody, CASP8 antibody, CASP-8 antibody, FLICE antibody, MACH antibody, Mch5 antibody, ALPS2B antibody, CAP4 antibody, Casp-8 antibody, MCH5 antibody, caspase-8 antibody, zgc:92075 antibody, caspase 8 L homeolog antibody, death related ced-3/Nedd2-like protein antibody, caspase 8 antibody, caspase-8 antibody, caspase-8-like antibody, caspase 8, apoptosis-related cysteine peptidase antibody, casp8.L antibody, LOC661095 antibody, CASP8 antibody, LOC5563818 antibody, casp8 antibody, LOC100222284 antibody, Casp8 antibody

Background: CAP4, FLICE, MACH, MCH5, Cysteinyl Aspartate Specific Proteinases 8, Apoptotic cysteine protease, FADD-homologous ICE/ced-3-like protease, MORT1-associated ced-3 homolog

Pathways: Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades