NG2 antibody (AA 559-776)

Catalog No. ABIN3201566

Product Details anti-NG2 Antibody

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Binding Specificity: AA 559-776

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Monoclonal Antibody to Chondroitin Sulfate Proteoglycan 4 (CSPG4)

Sequence: MGHHHHHHSG SEF-HG SLMVILEHTQ KPLGPEVFQA YDPDSACEGL TFQVLGTSSG LPVERRDQPG EPATEFSCRE LEAGSLVYVH RGGPAQDLTF RVSDGLQASP PATLKVVAIR PAIQIHRSTG LRLAQGSAMP ILPANLSVET NAVGQDVSVL FRVTGALQFG ELQKQGAGGV EGAEWWATQA FHQRDVEQGR VRYLSTDPQH HAYDTVENLA LEVQVGQEIL SNLSFP

Specificity: The antibody is a mouse monoclonal antibody raised against CSPG4. It has been selected for its ability to recognize CSPG4 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Chondroitin Sulfate Proteoglycan 4 (CSPG4) corresdonding to His559~Pro776 with N-terminal His Tag

Clone: 6#

Isotype: IgG1 kappa

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for NG2

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Alternative Name: Chondroitin Sulfate Proteoglycan 4 (MCSP Products)

Synonyms: HMW-MAA antibody, MCSP antibody, MCSPG antibody, MEL-CSPG antibody, MSK16 antibody, NG2 antibody, 4732461B14Rik antibody, AN2 antibody, Ng2 antibody, HSMCSGEN1 antibody, MCS antibody, chondroitin sulfate proteoglycan 4 antibody, sperm mitochondria associated cysteine rich protein antibody, CSPG4 antibody, Cspg4 antibody, SMCP antibody

Background: CSPG4, MSK16, HMW-MAA, MCSP, MCSPG, MEL-CSPG, NG2, Chondroitin Sulfate Proteoglycan 4, Melanoma chondroitin sulfate proteoglycan

Pathways: Glycosaminoglycan Metabolic Process