Caspase 4 antibody (AA 81-270)

Catalog No. ABIN3201614

Product Details anti-Caspase 4 Antibody

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Binding Specificity: AA 81-270

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Monoclonal Antibody to Caspase 4 (CASP4)

Sequence: MGHHHHHHSG S-KNSTSIKAPE ETVAGPDESV GSAATLKLCP HEEFLKLCKE RAGEIYPIKE RKDRTRLALI ICNTEFDHMP PRNGAALDIL GMKQLLEGLG YTVEVEEKLT ARDMESVLWK FAAREEHKSS DSTFLVFMSH GILDGICGTM HSEEEPDVLP YDTIFRTFNN RNCLSLKDKP KVIIVQACRG ANRGELWVSD

Specificity: The antibody is a mouse monoclonal antibody raised against CASP4. It has been selected for its ability to recognize CASP4 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Caspase 4 (CASP4) corresdonding to Lys81~Asp270 (Accession # Q5E9C1)

Clone: 2#

Isotype: IgG1 kappa

Application Details

Application Notes:

Western blotting: 0.5-5 μg/mL Immunohistochemistry: 5-30 μg/mL Immunocytochemistry: 5-30 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Caspase 4

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Alternative Name: Caspase 4 (CASP4 Products)

Synonyms: ICE(rel)II antibody, ICEREL-II antibody, ICH-2 antibody, Mih1/TX antibody, TX antibody, Casp11 antibody, CASP13 antibody, CASP1 antibody, CASP4 antibody, Caspl antibody, ich-3 antibody, Casp4 antibody, caspase 4 antibody, caspase 4, apoptosis-related cysteine peptidase antibody, caspase-4 antibody, CASP4 antibody, Casp4 antibody, LOC100731032 antibody

Background: ICE(rel)II, ICH2, TX, Protease ICH-2, Cysteinyl Aspartate Specific Proteinases 4, Apoptosis-Related Cysteine Peptidase

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity