COL4a5 antibody (AA 1461-1685)

Catalog No. ABIN3201692

Product Details anti-COL4a5 Antibody

Target: COL4a5 (COL4A5) (Collagen, Type IV, alpha 5 (COL4A5))

Binding Specificity: AA 1461-1685

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This COL4a5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Purpose: Monoclonal Antibody to Collagen Type IV Alpha 5 (COL4a5)

Sequence: MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- GFLITRHSQT TDAPQCPQGT LQVYEGFSLL YVQGNKRAHG QDLGTAGSCL RRFSTMPFMF CNINNVCNFA SRNDYSYWLS TPEPMPMSMQ PLKGQSIQPF ISRCAVCEAP AVVIAVHSQT IQIPHCPQGW DSLWIGYSFM MHTSAGAEGS GQALASPGSC LEEFRSAPFI ECHGRGTCNY YANSYSFWLA TVDVSDMFSK PQSETLKAGD LRTRISRCQV CMKRT

Specificity: The antibody is a mouse monoclonal antibody raised against COL4a5. It has been selected for its ability to recognize COL4a5 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Collagen Type IV Alpha 5 (COL4a5) corresdonding to Gly1461~Thr1685 with N-terminal His Tag

Clone: D2

Isotype: IgG1 kappa

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for COL4a5

Target: COL4a5 (COL4A5) (Collagen, Type IV, alpha 5 (COL4A5))

Alternative Name: Collagen Type IV Alpha 5 (COL4A5 Products)

Synonyms: ASLN antibody, ATS antibody, CA54 antibody, RGD1565499 antibody, col4a5 antibody, fj65e07 antibody, im:7157877 antibody, si:ch211-174g17.1 antibody, wu:fj65e07 antibody, collagen type IV alpha 5 chain antibody, collagen, type IV, alpha 5 antibody, collagen, type IV, alpha 1 antibody, COL4A5 antibody, Col4a5 antibody, col4a1 antibody, LOC100285389 antibody

Background: COL4-A5, ASLN, ATS, CA54, Collagen Alpha-5(IV)chain, Alport Syndrome

Pathways: Skeletal Muscle Fiber Development