WNT10B antibody (AA 30-322)

Catalog No. ABIN3202071

Product Details anti-WNT10B Antibody

Target: WNT10B (Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B))

Binding Specificity: AA 30-322

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNT10B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Purpose: Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 10B (WNT10B)

Specificity: The antibody is a rabbit polyclonal antibody raised against WNT10B. It has been selected for its ability to recognize WNT10B in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) corresdonding to Glu30~Pro322 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for WNT10B

Target: WNT10B (Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B))

Alternative Name: Wingless Type MMTV Integration Site Family, Member 10B (WNT10B Products)

Synonyms: WNT10B antibody, Xwnt-10b antibody, Xwnt10b antibody, wnt-12 antibody, Wnt12 antibody, SHFM6 antibody, WNT-12 antibody, Xwnt-10 antibody, wnt10 antibody, wnt10-A antibody, Zwnt[d] antibody, wnt10br antibody, wnt[d] antibody, Wnt family member 10B antibody, wingless-type MMTV integration site family, member 10B antibody, Wnt family member 10B S homeolog antibody, wingless-type MMTV integration site family, member 10b antibody, WNT10B antibody, wnt10b antibody, Wnt10b antibody, wnt10b.S antibody

Background: WNT-12, SHFM6

Pathways: WNT Signaling, Skeletal Muscle Fiber Development