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CD8 antibody (FITC)

CD8 Reactivity: Rat FACS Host: Mouse Monoclonal OX-8 FITC
Catalog No. ABIN320305
  • Target See all CD8 Antibodies
    CD8
    Reactivity
    • 227
    • 73
    • 26
    • 17
    • 12
    • 12
    • 9
    • 9
    • 9
    • 9
    • 8
    • 7
    • 7
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    Rat
    Host
    • 231
    • 54
    • 39
    Mouse
    Clonality
    • 268
    • 47
    • 2
    Monoclonal
    Conjugate
    • 97
    • 40
    • 32
    • 24
    • 11
    • 8
    • 6
    • 6
    • 6
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD8 antibody is conjugated to FITC
    Application
    • 273
    • 65
    • 64
    • 47
    • 45
    • 42
    • 30
    • 19
    • 17
    • 14
    • 14
    • 14
    • 8
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificity
    This antibody recognises the CD8 alpha cell surface antigen, expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells. Clone MRC OX-8 is suitable for use in in vitro blocking studies.
    Purification
    Ion Exchange Chromatography
    Immunogen
    Rat thymocyte membrane glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
    Clone
    OX-8
    Isotype
    IgG1
    Top Product
    Discover our top product CD8 Primary Antibody
  • Application Notes
    Flow cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL. This clone has been described reacting with paraffin-embedded material following PLP Fixation (periodatelysine paraformaldehyde).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    CD8
    Alternative Name
    CD8 (CD8 Products)
    Synonyms
    CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, CD8a molecule antibody, CD8A antibody
    Background
    The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
    Gene ID
    24930
    NCBI Accession
    NP_113726
    UniProt
    P07725
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