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CD11b antibody (FITC)

ITGAM Reactivity: Rat FACS Host: Mouse Monoclonal OX-42 FITC
Catalog No. ABIN320306
  • Target See all CD11b (ITGAM) Antibodies
    CD11b (ITGAM) (Integrin alpha M (ITGAM))
    Reactivity
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    Rat
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    Mouse
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    Monoclonal
    Conjugate
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    This CD11b antibody is conjugated to FITC
    Application
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    Flow Cytometry (FACS)
    Purification
    Affinity Chromatography on Protein G.
    Immunogen
    Rat resident peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSO/U myeloma cell line.
    Clone
    OX-42
    Isotype
    IgG2a
    Top Product
    Discover our top product ITGAM Primary Antibody
  • Application Notes
    Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL. Clone OX-42 has been reported as being suitable for use on paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Functionally, clone OX-42 inhibits complement mediated rosettes. We recommend the use of ABIN182004 for use in functional studies.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    Phosphate Buffered Saline, 1 % Bovine Serum Albumin, Sodium Azide 0.09 %
    Handling Advice
    Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    CD11b (ITGAM) (Integrin alpha M (ITGAM))
    Alternative Name
    CD11b (ITGAM Products)
    Synonyms
    CD11b/CD18 antibody, CR3 antibody, CR3A antibody, Cd11b antibody, F730045J24Rik antibody, Ly-40 antibody, MAC1 antibody, Mac-1 antibody, Mac-1a antibody, CD11B antibody, MAC-1 antibody, MAC1A antibody, MO1A antibody, SLEB6 antibody, C3bi antibody, integrin subunit alpha M antibody, integrin alpha M antibody, integrin, alpha M (complement component 3 receptor 3 subunit) antibody, integrin alpha-M antibody, ITGAM antibody, Itgam antibody, LOC100351865 antibody
    Background
    The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35 % of alveolar macrophages.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
    Gene ID
    10116
    UniProt
    Q63001
    Pathways
    Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
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