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CD45 antibody (Protein tyrosine Phosphatase, Receptor Type, C) (FITC)

Details for Product anti-PTPRC Antibody No. ABIN320317, Supplier: Log in to see
Antigen
  • B220
  • CD45
  • Cd45
  • cd45
  • CD45R
  • GP180
  • L-CA
  • Lca
  • LCA
  • loc
  • Ly-5
  • LY5
  • Lyt-4
  • PTPRC
  • RT7
  • si:dkeyp-47c5.1
  • T200
Reactivity
Rat (Rattus)
1231
728
217
143
62
52
41
40
24
21
18
8
5
5
5
4
3
3
2
1
1
1
1
1
Host
Mouse
1303
802
203
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD45 antibody is conjugated to FITC
285
229
171
103
49
48
47
41
40
31
27
27
27
26
25
24
24
24
24
21
17
17
16
16
14
14
10
9
9
7
6
6
6
5
5
5
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1847
528
502
476
446
381
327
226
189
49
46
40
26
12
11
10
7
7
6
6
4
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Immunogen Rat thymocyte membrane glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Clone OX-1
Isotype IgG1
Specificity This antibody recognises CD45. Antibodies recognising a common epitope on all of these isoforms are termed CD45, whilst those recognising only individual isoforms are termed CD45RA, CD45RO etc. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes.
Alternative Name CD45 / LCA (PTPRC Antibody Abstract)
Background The leucocyte common antigen consists of a family of heavily glycosylated membrane glycoproteins of molecular weight 180 - 240 kDa. CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Gene ID 24699
NCBI Accession NP_001103357
UniProt P04157
Application Notes Flow Cytometry: Use 10 μL of 1/50 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Sato, Balla, Otterbein, Smith, Brouard, Lin, Csizmadia, Sevigny, Robson, Vercellotti, Choi, Bach, Soares: "Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 6, pp. 4185-94, 2001 (PubMed).

Murakami, Sato, Nagasawa, Yamashita: "Regulation of mast cell signaling through high-affinity IgE receptor by CD45 protein tyrosine phosphatase." in: International immunology, Vol. 12, Issue 2, pp. 169-76, 2000 (PubMed).

Martín, Molina, Bricio, Mampaso: "Passive dual immunization against tumour necrosis factor-alpha (TNF-alpha) and IL-1 beta maximally ameliorates acute aminonucleoside nephrosis." in: Clinical and experimental immunology, Vol. 99, Issue 2, pp. 283-8, 1995 (PubMed).

Woollett, Barclay, Puklavec, Williams: "Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes." in: European journal of immunology, Vol. 15, Issue 2, pp. 168-73, 1985 (PubMed).

Sunderland, McMaster, Williams: "Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes." in: European journal of immunology, Vol. 9, Issue 2, pp. 155-9, 1979 (PubMed).