CrkL antibody (AA 1-303)

Catalog No. ABIN3203222

Product Details anti-CrkL Antibody

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Binding Specificity: AA 1-303

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CrkL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Crk Like Protein (CRKL)

Specificity: The antibody is a rabbit polyclonal antibody raised against CRKL. It has been selected for its ability to recognize CRKL in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Crk Like Protein (CRKL) corresdonding to Met1~Glu303 (Accession # P46109)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:290-1200 Immunohistochemistry: 5-20 μg/mL

1:29-120 Immunocytochemistry: 5-20 μg/mL

1:29-120 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CrkL

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Alternative Name: Crk Like Protein (CRKL Products)

Synonyms: CRKL antibody, wu:fa55g05 antibody, wu:fj60e01 antibody, zgc:63469 antibody, 1110025F07Rik antibody, AA589403 antibody, AI325100 antibody, Crkol antibody, CRK like proto-oncogene, adaptor protein antibody, v-crk avian sarcoma virus CT10 oncogene homolog-like antibody, CRKL antibody, crkl antibody, Crkl antibody

Background: V-Crk Sarcoma Virus CT10 Oncogene Homolog(avian)-Like

Pathways: Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor