Inhibitory Subunit of NF-KappaB epsilon (AA 207-440) antibody

Catalog No. ABIN3203929

Product Details

Target: Inhibitory Subunit of NF-KappaB epsilon

Binding Specificity: AA 207-440

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Polyclonal Antibody to Inhibitory Subunit Of NF Kappa B Epsilon (IkBe)

Specificity: The antibody is a rabbit polyclonal antibody raised against IkBe. It has been selected for its ability to recognize IkBe in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Inhibitory Subunit Of NF Kappa B Epsilon (IkBe) corresdonding to Arg207~Pro440 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: Inhibitory Subunit of NF-KappaB epsilon

Alternative Name: Inhibitory Subunit Of NF Kappa B Epsilon

Synonyms: IKBE antibody, NFKB inhibitor epsilon antibody, nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, epsilon antibody, NFKBIE antibody, Nfkbie antibody

Background: NFKBIE, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor,Epsilon