PDGFRA antibody (AA 170-387)

Catalog No. ABIN1869734

Product Details anti-PDGFRA Antibody

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Binding Specificity: AA 170-387

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDGFRA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Purpose: Polyclonal Antibody to Platelet Derived Growth Factor Receptor Alpha (PDGFRa)

Specificity: The antibody is a rabbit polyclonal antibody raised against PDGFRa. It has been selected for its ability to recognize PDGFRa in immunohistochemical staining and western blotting.

Cross-Reactivity: Cow, Dog, Goat, Guinea Pig, Mouse, Pig, Rat, Sheep

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Platelet Derived Growth Factor Receptor Alpha (PDGFRa) corresdonding to Ala170~Glu387 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PDGFRA

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Alternative Name: Platelet Derived Growth Factor Receptor Alpha (PDGFRA Products)

Synonyms: AI115593 antibody, CD140a antibody, Pdgfr-2 antibody, APDGFR antibody, PDGFACE antibody, CD140A antibody, PDGFR-2 antibody, PDGFR2 antibody, RHEPDGFRA antibody, PDGFR antibody, PDGFR-A antibody, platelet derived growth factor receptor alpha antibody, platelet derived growth factor receptor, alpha polypeptide antibody, platelet-derived growth factor receptor, alpha polypeptide L homeolog antibody, platelet-derived growth factor receptor, alpha polypeptide antibody, PDGFRA antibody, Pdgfra antibody, pdgfra.L antibody

Background: CD140a, PDGFR2, Rhe-PDGFRA, CD140 antigen-like family member A, Platelet-derived growth factor receptor 2, Alpha-type platelet-derived growth factor receptor

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling