NFkB antibody (AA 42-367)

Catalog No. ABIN3206002

Product Details anti-NFkB Antibody

Target: NFkB (Nuclear Factor kappa B (NFkB))

Binding Specificity: AA 42-367

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFkB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Nuclear Factor Kappa B (NFkB)

Specificity: The antibody is a rabbit polyclonal antibody raised against NFkB. It has been selected for its ability to recognize NFkB in immunohistochemical staining and western blotting.

Cross-Reactivity: Cow, Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Nuclear Factor Kappa B (NFkB) corresdonding to Pro42~Met367 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-NFkB antibody (ABIN3206002)

Seetha, Devaraj, Sudhandiran: "Indomethacin and juglone inhibit inflammatory molecules to induce apoptosis in colon cancer cells." in: Journal of biochemical and molecular toxicology, Vol. 34, Issue 2, pp. e22433, (2020) (PubMed).

Target Details for NFkB

Target: NFkB (Nuclear Factor kappa B (NFkB))

Alternative Name: Nuclear Factor Kappa B (NFkB Products)

Synonyms: EBP-1 antibody, KBF1 antibody, NF-kB1 antibody, NF-kappa-B antibody, NF-kappaB antibody, NFKB-p105 antibody, NFKB-p50 antibody, NFkappaB antibody, p105 antibody, p50 antibody, NF-kB antibody, NF-KB1 antibody, NF-kappaB1 antibody, p50/p105 antibody, nuclear factor kappa B subunit 1 antibody, nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 antibody, NFKB1 antibody, Nfkb1 antibody

Background: NF-KB1, EBP-1, KBF1, NF-Kappa-B, NFKB-P105, NFKB-P50, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1, Nuclear factor NF-kappa-B p105 subunit

Pathways: Ubiquitin Proteasome Pathway, S100 Proteins