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C3 antibody (AA 23-300)

C3 Reactivity: Pig WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1862261
  • Target See all C3 Antibodies
    C3 (Complement Component 3 (C3))
    Binding Specificity
    • 15
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 23-300
    Reactivity
    • 94
    • 57
    • 35
    • 6
    • 4
    • 4
    • 3
    • 3
    • 1
    Pig
    Host
    • 72
    • 47
    • 28
    • 8
    • 7
    • 6
    Rabbit
    Clonality
    • 129
    • 38
    Polyclonal
    Conjugate
    • 75
    • 24
    • 24
    • 15
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    This C3 antibody is un-conjugated
    Application
    • 110
    • 54
    • 40
    • 35
    • 34
    • 30
    • 30
    • 29
    • 23
    • 18
    • 14
    • 13
    • 13
    • 9
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Purpose
    Polyclonal Antibody to Complement Component 3 (C3)
    Specificity
    The antibody is a rabbit polyclonal antibody raised against C3. It has been selected for its ability to recognize C3 in immunohistochemical staining and western blotting.
    Purification
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogen
    Recombinant Complement Component 3 (C3) corresdonding to Asp23~Thr300
    Isotype
    IgG
    Top Product
    Discover our top product C3 Primary Antibody
  • Application Notes

    Western blotting: 0.1-2 μg/mL

    Immunohistochemistry: 5-20 μg/mL

    Immunocytochemistry: 5-20 μg/mL

    Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Expiry Date
    24 months
  • Zhang, Wang, Wang, Jin, Zhao, Xiong, Zhang, Mou, Li, Yang, Dai: "Generation of complement protein C3 deficient pigs by CRISPR/Cas9-mediated gene targeting." in: Scientific reports, Vol. 7, Issue 1, pp. 5009, (2019) (PubMed).

  • Target
    C3 (Complement Component 3 (C3))
    Alternative Name
    Complement Component 3 (C3 Products)
    Synonyms
    AHUS5 antibody, ARMD9 antibody, ASP antibody, C3a antibody, C3b antibody, CPAMD1 antibody, AI255234 antibody, HSE-MSF antibody, Plp antibody, C3 antibody, C3-1 antibody, sb:cb26 antibody, si:dkey-76b14.4 antibody, wu:fi34b03 antibody, wu:fj86e11 antibody, C3d antibody, asp antibody, cpamd1 antibody, c3 antibody, complement C3 antibody, complement component 3 antibody, complement component c3a, duplicate 1 antibody, complement component 3 L homeolog antibody, complement component C3 antibody, complement C3-like antibody, C3 antibody, c3a.1 antibody, c3.L antibody, LOC443475 antibody, c3 antibody, c3-1 antibody, LOC100060539 antibody
    Background
    ASP, CPAMD1, Complement Protein C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3a anaphylatoxin, Acylation stimulating protein
    Pathways
    Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling
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