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Perforin 1 antibody (AA 40-355)

PRF1 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN3210093
  • Target See all Perforin 1 (PRF1) Antibodies
    Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))
    Binding Specificity
    • 8
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 40-355
    Reactivity
    • 80
    • 26
    • 10
    • 7
    • 6
    • 5
    • 2
    • 1
    • 1
    • 1
    Mouse
    Host
    • 57
    • 41
    • 4
    Rabbit
    Clonality
    • 58
    • 44
    Polyclonal
    Conjugate
    • 58
    • 13
    • 11
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Perforin 1 antibody is un-conjugated
    Application
    • 50
    • 40
    • 30
    • 20
    • 15
    • 10
    • 7
    • 7
    • 6
    • 5
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Purpose
    Polyclonal Antibody to Perforin 1 (PRF1)
    Specificity
    The antibody is a rabbit polyclonal antibody raised against PRF1. It has been selected for its ability to recognize PRF1 in immunohistochemical staining and western blotting.
    Cross-Reactivity
    Human, Rat
    Purification
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogen
    Recombinant Perforin 1 (PRF1) corresdonding to Val40~Lys355 with N-terminal His and GST Tag
    Isotype
    IgG
    Top Product
    Discover our top product PRF1 Primary Antibody
  • Application Notes

    Western blotting: 0.5-2 μg/mL

    Immunohistochemistry: 5-20 μg/mL

    Immunocytochemistry: 5-20 μg/mL

    Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
    Preservative
    ProClin
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Expiry Date
    24 months
  • Target
    Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))
    Alternative Name
    Perforin 1 (PRF1 Products)
    Synonyms
    FLH2 antibody, HPLH2 antibody, P1 antibody, PFN1 antibody, PFP antibody, PRF1 antibody, Pfn antibody, Pfp antibody, Prf-1 antibody, Cyta antibody, RATCYTA antibody, LOC443187 antibody, perforin antibody, prf1 antibody, cytolysin antibody, perforin-1 antibody, perforin-1-like antibody, perforin 1 antibody, perforin 1 (pore forming protein) antibody, perforin antibody, perforin 1 L homeolog antibody, PRF1 antibody, Prf1 antibody, LOC443187 antibody, prf1 antibody, prf1.L antibody
    Background
    FLH2, HPLH2, P1, PFP, Pore Forming Protein, Cytolysin, Lymphocyte pore-forming protein
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis
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