DDR2 antibody (Discoidin Domain Receptor tyrosine Kinase 2) (AA 1-399)

Details for Product anti-DDR2 Antibody No. ABIN3218115, Supplier: Log in to see
Antigen
  • DDR2
  • ddr2
  • si:ch211-193c2.1
  • AW495251
  • Ntrkr3
  • tyro10
  • MIG20a
  • NTRKR3
  • TKT
  • TYRO10
  • Tyro10
  • discoidin domain receptor tyrosine kinase 2
  • discoidin domain receptor family, member 2b
  • discoidin domain receptor family, member 2
  • DDR2
  • ddr2b
  • LOC100356982
  • Ddr2
Alternatives
anti-Mouse (Murine) DDR2 antibody for ELISA
Epitope
AA 1-399
15
13
12
11
8
6
4
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
91
35
5
1
1
Host
Rabbit
76
40
Clonality
Polyclonal
Conjugate
This DDR2 antibody is un-conjugated
4
4
4
4
4
4
2
2
2
2
2
2
2
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
85
58
38
22
18
12
5
3
2
1
1
Options
Supplier
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Immunogen Recombinant Mouse DDR2 protein (ABIN2007949)
Isotype IgG
Specificity Mouse DDR2
Characteristics Produced in rabbits immunized with purified, recombinant Mouse DDR2 (rM DDR2, ABIN2007949 NP_072075.2, Met 1-Arg 399,. DDR2 specific IgG was purified by Mouse DDR2 affinity chromatography.
Purification purified by affinity chromatography
Sterility 0.2 μm filtered
Alternative Name DDR2 (DDR2 Antibody Abstract)
Background Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple- helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.
NCBI Accession NP_072075.2
Research Area Cardiovascular, Cardiogenesis
Pathways RTK Signaling
Application Notes ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse DDR2. The detection limit for Mouse DDR2 is approximately 0.039 ng/well.
IHC-P: 0.1-2 μg/mL
Restrictions For Research Use only
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DDR2 antibody (Discoidin Domain Receptor tyrosine Kinase 2) (AA 1-399) (ABIN3218115) anti-Discoidin Domain Receptor tyrosine Kinase 2 (DDR2) (AA 1-399) antibody
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DDR2 antibody (Discoidin Domain Receptor tyrosine Kinase 2) (AA 1-399) (ABIN3218115) anti-Discoidin Domain Receptor tyrosine Kinase 2 (DDR2) (AA 1-399) antibody (Image 2)
Background publications Zhang, Ding, Zhang, Su, Li, Liu, Ma, Yao: "Expression of discoidin domain receptor 2 (DDR2) extracellular domain in pichia pastoris and functional analysis in synovial fibroblasts and NIT3T3 cells." in: Molecular and cellular biochemistry, Vol. 290, Issue 1-2, pp. 43-53, 2006 (PubMed).

Leitinger, Steplewski, Fertala: "The D2 period of collagen II contains a specific binding site for the human discoidin domain receptor, DDR2." in: Journal of molecular biology, Vol. 344, Issue 4, pp. 993-1003, 2004 (PubMed).