FAS antibody (Extracellular Domain) (PE)

Catalog No. ABIN323507

Product Details anti-FAS Antibody (PE)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Binding Specificity: Extracellular Domain

Reactivity: Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAS antibody is conjugated to PE

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Specificity: Recognizes feline Fas extracellular domain. Species sequence homology: human 68 % , mouse 65 %, porcine 53 %, and rat 58 %. Does not cross-react with rhFas, rmFas, or rrFas.

Purification: Protein G purified

Immunogen: NS0-derived recombinant feline rfeFas extracellular domain.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Flow cytometry, Western blot and Direct ELISA. Flow cytometry: 25 μg/mL and add 10il of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Western Blot: 1-2 μg/mL. the detection limit for rfeFas is approximately 25 ng/lane under non-reducing conditions. It is not recommended to use this antibody under reducing conditions. Chemiluminescent detection with WesternGlo Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Direct ELISA: 0.5-1.0 μg/mL The detection limit for rfeFas is approximately 3 ng/well. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Cat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized powder from PBS, 5 % trehalose. Reconstitute, sterile PBS to a concentration of 0.5 mg/mL.

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: CD95 / FAS (FAS Products)

Synonyms: ALPS1A antibody, APO-1 antibody, APT1 antibody, CD95 antibody, FAS1 antibody, FASTM antibody, TNFRSF6 antibody, AI196731 antibody, APO1 antibody, TNFR6 antibody, Tnfrsf6 antibody, lpr antibody, Fas cell surface death receptor antibody, Fas (TNF receptor superfamily member 6) antibody, FAS antibody, Fas antibody, fas antibody

Background: Name/Gene ID: FAS
Family: TNF Receptor

Synonyms: FAS, ALPS1A, APO-1, APT1, Apo-1 antigen, APO-1 cell surface antigen, Apo11 antigen, Apoptosis antigen 1, CD95 antigen, Delta Fas/APO-1/CD95, Fas AMA, FAS1, Fasl receptors, FASLG receptor, FASTM, FAS 827dupA, Fasl receptor, TNFRSF6, CD95

Gene ID: 355

UniProt: P25445

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity