14-3-3 (YWHA) antibody

Antigen: 14-3-3 (YWHA)

Synonyms: MDS, MDCR, KCIP-1, 14-3-3E, FLJ45465, AU019196, 14-3-3e, 14-3-3, 14-3-3-e, CT24092, D14-3-3e, D14-3-3epsilon, EK3-5, PAR5, Par-5, SR3-9, Su(Raf)3B, anon-WO0172774.141, anon-WO02059370.52, l(3)j2B10, DmelCG31196, CG31196, LOC443300, YWHAE, mds, mdcr, kcip-1, MGC89915, 14-3-3epsilon, LOC581376, Ywhae, ACYPI002517

Clonality: Monoclonal (22-IID8B)

Host: Mouse

Reactivity: Human

Application: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN331518

Additional Information

Alternative name: 14-3-3

Immunogen: Cells, Lysed transformed human amnion cells (AMA).

Isotype: IgG  (Matching secondary antibodies)

Clone: 22-IID8B

Description: Gene: 14-3-3

Specificity: Detects ~29-30 kD proteins, corresponding to the apparent molecular mass of the beta and zeta isoforms of 14-3-3 on SDS-PAGE immunoblots, in samples from human, mouse, rat, monkey, rabbit, dog, sheep, pig and bovine origins. This antibody is suitable as a Golgi marker as the epitopes recognized are on the cytoplasmic face of the Golgi membrane. It also reacts specifically with two members of the 14-3-3 protein family: the acidic human IEF58 (~29 kD) and IEF57a (~30 kD), which correspond to 14-3-3 beta and zeta, respectively. Predicted Reactivities: Cow (Bovine),Dog (Canine),Monkey,Mouse (Murine),Pig (Porcine),Rabbit,Rat (Rattus),Sheep (Ovine).

Synonyms: MDS, MDCR, KCIP-1, 14-3-3E, FLJ45465, AU019196, 14-3-3e, 14-3-3, 14-3-3-e, CT24092, D14-3-3e, D14-3-3epsilon, EK3-5, PAR5, Par-5, SR3-9, Su(Raf)3B, anon-WO0172774.141, anon-WO02059370.52, l(3)j2B10, DmelCG31196, CG31196, LOC443300, YWHAE, mds, mdcr, kcip-1, MGC89915, 14-3-3epsilon, LOC581376, Ywhae, ACYPI002517

Application Details

Application Notes: ICC, IHC (1:50), WB

Purification: Protein G Column

Buffer: PBS, pH 7.2, 0.1 mM PMSF, 0.09% azide in 50% glycerol

Storage: Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

Restrictions: For Research Use only