PKC alpha antibody

Catalog No. ABIN331530

Product Details anti-PKC alpha Antibody

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Reactivity: Human, Mouse, Rat, Cow, Dog, Fish

Host: Mouse

Clonality: Monoclonal

Conjugate: This PKC alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificity: Detects an ~80kD protein, corresponding to the apparent molecular mass of PKC alpha on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, fish (rainbow trout), guinea pig, monkey, pig, rabbit and sheep origins. The epitope of this antibody is within the variable region V3 of PKC alpha (the site of limited proteolysis on the native enzyme). This antibody is specific for PKC alpha and does not detect the other PKC isoforms.

Purification: Protein G purified

Immunogen: Bovine brain PKCa.

Clone: MC5

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (10 μg/mL), IHC (1:50), IP (12.5 μg/mL), WB (1 μg/mL)

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS 0.1 mM PMSF, 50 % glycerol

Preservative: PMSF

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for PKC alpha

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Alternative Name: PRKCA / PKC-alpha (PKCa Products)

Synonyms: pkc-alpha antibody, pkcalpha antibody, im:7139045 antibody, si:dkey-8l19.1 antibody, LOC100219195 antibody, AAG6 antibody, PKC-alpha antibody, PKCA antibody, PRKACA antibody, AI875142 antibody, Pkca antibody, PKRCA antibody, protein kinase C alpha antibody, protein kinase C, alpha L homeolog antibody, protein kinase C, alpha antibody, PRKCA antibody, prkca.L antibody, prkca antibody, Prkca antibody

Background: Name/Gene ID: PRKCA
Subfamily: PKC
Family: Protein Kinase

Synonyms: PRKCA, Aging-associated gene 6, AAG6, Alpha PKC, PKC-A, Pkcalpha, Protein kinase C alpha type, PKC Alpha, PKC-alpha, PKCA, Protein kinase c alpha, Protein kinase C, alpha

Gene ID: 5578

Pathways: WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling