serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 2 (SERPINF2) antibody

Antigen: serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 2 (SERPINF2)

Synonyms: AAP, API, PLI, A2AP, ALPHA-2-PI, Pli, AI747498, Serpimf2, SERPINF2, SERPINF1, serpin

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN334343

Additional Information

Alternative name: Alpha2-antiplasmin

Gene ID: 5345

Immunogen: Peptide with sequence C-KDFLQSLKGFPRGDK, from the internal region of the protein sequence according to NP_000925.2

Description: All names and symbols: SERPINF2, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2, A2AP, AAP, ALPHA-2-PI, API, PLI, alpha-2-plasmin inhibitor, serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2. Official Symbol: SERPINF2. RefSeq number(s): NP_000925.2.

Specificity: Expected from sequence similarity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx. 70kDa band observed in Human Duodenum and Ileum lysates (calculated MW of 54.6kDa according to NP_000925.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lee et al, Blood. 2004 May 15,103(10):3783-8., PMID: 14751930). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Liver shows potty cytoplasm staining in hepatocytes. Recommended concentration, 3-5µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Matsuno, Okada, Ueshima et al.: "Alpha2-antiplasmin plays a significant role in acute pulmonary embolism." in: Journal of thrombosis and haemostasis : JTH, Vol. 1, Issue 8, pp. 1734-9, 2003 (PubMed).