Angptl4 (mouse) antibody

Antigen: Angptl4 (mouse)

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN334345

Additional Information

Gene ID: 57875 (mouse), 362850 (rat)

Immunogen: C-HLDNGVDKTSRGKK

Description: All names and symbols: Angptl4, angiopoietin-like 4, ARP4, FIAF, HFARP, Bk89, Ng27, PGAR, pp1158, PPARG, fibrinogen/angiopoietin-related protein, major histocompatibility complex region NG27. Official Symbol: ANGPTL4. RefSeq number(s): NP_065606.1

Specificity: Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 26kDa band in Mouse Liver and Rat Liver lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.5kDa according to Mouse NP_065606.1. The 26kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Lichtenstein, Berbee, van Dijk et al.: "Angptl4 upregulates cholesterol synthesis in liver via inhibition of LPL- and HL-dependent hepatic cholesterol uptake." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 27, Issue 11, pp. 2420-7, 2007 (PubMed).