Calcium Channel, Voltage-Dependent, beta 4 Subunit (CACNB4) (internal) antibody

Antigen: Calcium Channel, Voltage-Dependent, beta 4 Subunit (CACNB4)

Synonyms: EA5, EJM, CAB4, EIG9, EJM4, EJM6, CACNLB4, lh, Cchb4, MGC31529, lethargic, 3110038O15Rik, CACNB4, cacnb4.1

Epitope: internal

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334367

Additional Information

Alternative name: CACNB4 (internal)

Gene ID: 785, 12298 (mouse), 58942 (rat)

Immunogen: C-DYPDSYQDTYKPH

Description: All names and symbols: CACNB4, calcium channel, voltage-dependent, beta 4 subunit, CAB4, CACNLB4, EA5, EJM , dihydropyridine-sensitive L-type, calcium channel beta-4 subunit, voltage dependent calcium channel beta 4 subunit. Official Symbol: CACNB4. RefSeq number(s): NP_001005747.1, NP_000717.2, NP_001005746.1

Specificity: This antibody is expected to recognise all three reported isoforms (NP_001005747.1, NP_000717.2, NP_001005746.1). Human, Mouse, Rat, Dog 520 aa, 502 aa, 486 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 55-60kDa band observed in Human Bone Marrow lysates (calculated MW of 54.7kDa according to NP_001005747.1 and 58.2kDa according to NP_000717.2). Recommended concentration: 0.2-0.6 µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334367 (0.2µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Escayg, Jones, Kearney et al.: "Calcium channel beta 4 (CACNB4): human ortholog of the mouse epilepsy gene lethargic." in: Genomics, Vol. 50, Issue 1, pp. 14-22, 1999 (PubMed).