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Collagen, Type XI, alpha 1 (COL11A1) (Internal Region) antibody

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Synonyms wu:fb34e11, wu:fc11b03, zgc:158335, col11a1, Col11a1, CO11A1, COLL6, STL2, C530001D20Rik, cho
Internal Region
(13), (1), (1), (1), (1)
(27), (4), (3), (1), (1), (1), (1), (1), (1)
(17), (15)
(3), (3), (3), (1), (1), (1)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB)
(23), (16), (2), (1)
Pubmed 1 reference available
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Quantity 100 μg
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Immunogen Peptide with sequence C-EDYDSQRKNSEDTLY, from the internal region of the protein sequence according to NP_001845.3, NP_542196.2 , NP_542197.2.
Blocking Peptide Blocking peptide for this product available: ABIN368968
Specificity This antibody is expected to recognise all three reported isoforms (NP_001845.3, NP_542196.2 and NP_542197.2).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name COL11A1 (COL11A1 Antibody Abstract)
Background Synonyms: COL11A1, collagen, type XI, alpha 1, CO11A, COLL6, STL2, alpha 1 type XI collagen, collagen XI alpha 1, collagen XI, alpha-1 polypeptide
Gene ID 1301
NCBI Accession NP_001845, NP_542197, NP_542196
Research Area Signaling, Extracellular Matrix
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments gave an approx 28 kDa band in lysates of cell lines A431, A549, HEK293 after 0.5 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 181 kDa according to NP_001845.3. The 28 kDa band was successfully blocked by incubation with the immunizing peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Van Camp, Snoeckx, Hilgert et al.: "A new autosomal recessive form of Stickler syndrome is caused by a mutation in the COL9A1 gene." in: American journal of human genetics, Vol. 79, Issue 3, pp. 449-57, 2006 (PubMed).

Catalog No. ABIN334377
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