Procollagen, Type Xi, alpha 1 (COL11A1) antibody

Antigen: Procollagen, Type Xi, alpha 1 (COL11A1)

Synonyms: cho, C530001D20Rik, COL11A1, COLL11A1, Col11a1, wu:fb54e07, col11a1

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN334377

Additional Information

Alternative name: COL11A1

Gene ID: 1301

Immunogen: C-EDYDSQRKNSEDTLY

Description: All names and symbols: COL11A1, collagen, type XI, alpha 1, CO11A, COLL6, STL2 , alpha 1 type XI collagen, collagen XI alpha 1, collagen XI, alpha-1 polypeptide. Official Symbol: COL11A1. RefSeq number(s): NP_001845.3, NP_542196.2 , NP_542197.2

Specificity: This antibody is expected to recognise all three reported isoforms (NP_001845.3, NP_542196.2 and NP_542197.2). Human 1806 aa, 1818 aa, 1767 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Preliminary experiments gave an approx 28kDa band in lysates of cell lines A431, A549, HEK293 after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 181kDa according to NP_001845.3. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Extracellular Matrix

Restrictions: For Research Use only

Publications

Publications: Van Camp, Snoeckx, Hilgert et al.: "A new autosomal recessive form of Stickler syndrome is caused by a mutation in the COL9A1 gene." in: American journal of human genetics, Vol. 79, Issue 3, pp. 449-57, 2006 (PubMed).