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DACH1 antibody (Dachshund Homolog 1 (Drosophila)) (Internal Region)

Details for Product anti-DACH1 Antibody No. ABIN334382, Supplier: Log in to see
Antigen
  • AI182278
  • Dac
  • DACH
  • Dach
  • dach
  • E130112M23Rik
  • zgc:158869
Epitope
Internal Region
17
15
10
3
2
2
1
1
1
1
Reactivity
Human
67
30
25
2
1
1
1
Host
Goat
55
12
Clonality
Polyclonal
Conjugate
This DACH1 antibody is un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
51
29
12
11
4
3
2
1
Supplier
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Immunogen Peptide with sequence SPVENTPQNNECK, from the internal region of the protein sequence according to NP_542937.1, NP_542938.1, NP_004383.2.
Blocking Peptide Blocking peptide for this product available: ABIN370462
Specificity This antibody is expected to recognise all three reported isoforms(NP_542937.1, NP_542938.1, NP_004383.2).
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Dachshund homolog 1 (DACH1 Antibody Abstract)
Background Synonyms: OTTHUMP00000040839, dachshund homolog 1 (Drosophila), DACH1, RP11-512J14.1, DACH, FLJ10138, dachshund homolog 1
Gene ID 1602, 13134
NCBI Accession NP_542938, NP_542937, NP_004383
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 75 and 52 kDa bands observed in Human Kidney lysates (calculated MW of 73.0 kDa according to NP_542937.1 and of 51.9 kDa according to NP_004383.2). Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-DACH1 antibody (Dachshund Homolog 1 (Drosophila)) (Internal Region) (ABIN334382) ABIN334382 (0.1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). ...
Background publications Wu, Yang, Wang, Davoli, DAmico, Li, Cveklova, Kozmik, Lisanti, Russell, Cvekl, Pestell: "DACH1 inhibits transforming growth factor-beta signaling through binding Smad4." in: The Journal of biological chemistry, Vol. 278, Issue 51, pp. 51673-84, 2003