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DACH2 antibody (Dachshund Homolog 2 (Drosophila)) (Internal Region)

Details for Product anti-DACH2 Antibody No. ABIN334383, Supplier: Log in to see
Antigen
  • 9430028N04Rik
  • cb907
  • RGD1561687
Epitope
Internal Region
16
15
14
4
2
1
Reactivity
Mouse (Murine)
52
25
15
3
2
2
1
1
1
1
Host
Goat
42
15
1
Clonality
Polyclonal
Conjugate
This DACH2 antibody is un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
42
29
11
4
4
1
1
1
Supplier
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Immunogen Peptide with sequence C-TRKQAVNSSRPGR, from the internal region of the protein sequence according to NP_444511.1.
Blocking Peptide Blocking peptide for this product available: ABIN369987
Cross-Reactivity Human
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Dachshund homolog 2 / DACH2 (DACH2 Antibody Abstract)
Background Synonyms: DACH2, dachshund homolog 2 (Drosophila), RP11-345E19.1, FLJ31391, MGC138545, dachshund 2
Gene ID 117154, 93837
NCBI Accession NP_444511
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 65-70 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 65.3 kDa according to human NP_444511.1 and of 68.6 kDa according to mouse NP_291083.1). Recommended concentration: 0.03-0.1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-DACH2 antibody (Dachshund Homolog 2 (Drosophila)) (Internal Region) (ABIN334383) ABIN334383 (0.03µg/ml) staining of NIH3T3 cell lysate (35µg protein in RIPA buffer). ...
Background publications Wu, Yang, Wang, Davoli, DAmico, Li, Cveklova, Kozmik, Lisanti, Russell, Cvekl, Pestell: "DACH1 inhibits transforming growth factor-beta signaling through binding Smad4." in: The Journal of biological chemistry, Vol. 278, Issue 51, pp. 51673-84, 2003 (PubMed).