Dachshund Homolog 2 (Drosophila) (DACH2) (Internal Region) antibody

Details for Product No. ABIN334383
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Antigen
Synonyms cb907, 9430028N04Rik, RGD1561687
Epitope
Internal Region
(7), (7), (5), (3), (1)
Reactivity
Mouse (Murine)
(29), (17), (15), (14), (13), (12), (12), (2), (1), (1), (1)
Host
Goat
(25), (5), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(22), (10), (9), (3), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN334383
374.27 $
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Immunogen Peptide with sequence C-TRKQAVNSSRPGR, from the internal region of the protein sequence according to NP_444511.1.
Cross-Reactivity Human
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Dachshund homolog 2 / DACH2
Background Synonyms: DACH2, dachshund homolog 2 (Drosophila), RP11-345E19.1, FLJ31391, MGC138545, dachshund 2
Gene ID 117154, 93837
NCBI Accession NP_444511
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 65-70 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 65.3 kDa according to human NP_444511.1 and of 68.6 kDa according to mouse NP_291083.1). Recommended concentration: 0.03-0.1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Dachshund Homolog 2 (Drosophila) (DACH2) (Internal Region) antibody ABIN334383 (0.03µg/ml) staining of NIH3T3 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Wu, Yang, Wang et al.: "DACH1 inhibits transforming growth factor-beta signaling through binding Smad4." in: The Journal of biological chemistry, Vol. 278, Issue 51, pp. 51673-84, 2003 (PubMed).

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