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Dachshund Homolog 2 (Drosophila) (DACH2) (Internal Region) antibody

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Antigen
Synonyms cb907, 9430028N04Rik, RGD1561687
Epitope
Internal Region
(16), (15), (12), (5), (2), (1)
Reactivity
Mouse (Murine)
(50), (24), (15), (3), (2), (2), (1), (1), (1), (1)
Host
Goat
(39), (15), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(40), (29), (11), (3), (3), (1)
Pubmed 1 reference available
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Quantity 100 μg
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Immunogen Peptide with sequence C-TRKQAVNSSRPGR, from the internal region of the protein sequence according to NP_444511.1.
Blocking Peptide Blocking peptide for this product available: ABIN369987
Cross-Reactivity Human
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Dachshund homolog 2 / DACH2 (DACH2 Antibody Abstract)
Background Synonyms: DACH2, dachshund homolog 2 (Drosophila), RP11-345E19.1, FLJ31391, MGC138545, dachshund 2
Gene ID 117154, 93837
NCBI Accession NP_444511
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 65-70 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 65.3 kDa according to human NP_444511.1 and of 68.6 kDa according to mouse NP_291083.1). Recommended concentration: 0.03-0.1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Image no. 1 for anti-Dachshund Homolog 2 (Drosophila) (DACH2) (Internal Region) antibody (ABIN334383) ABIN334383 (0.03µg/ml) staining of NIH3T3 cell lysate (35µg protein in RIPA buffer). ...
Background publications Wu, Yang, Wang et al.: "DACH1 inhibits transforming growth factor-beta signaling through binding Smad4." in: The Journal of biological chemistry, Vol. 278, Issue 51, pp. 51673-84, 2003 (PubMed).

Catalog No. ABIN334383
$221.76
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