HCST antibody (Hematopoietic Cell Signal Transducer) (C-Term)

Details for Product anti-HCST Antibody No. ABIN334384, Supplier: Log in to see
Antigen
  • HCST
  • DAP10
  • KAP10
  • PIK3AP
  • Dap10
  • hematopoietic cell signal transducer
  • HCST
  • hcst
  • Hcst
Epitope
C-Term
12
2
1
1
1
Reactivity
Human
30
1
1
1
Host
Goat
15
15
Clonality
Polyclonal
Conjugate
This HCST antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Method Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
15
13
12
3
1
Options
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Immunogen Peptide with sequence C-RSPAQEDGKVYIN, from the C Terminus of the protein sequence according to NP_055081.1, NP_001007470.1.
Blocking Peptide Blocking peptide for this product available: ABIN369830
Specificity This antibody is expected to recognise isoform 1 (NP_055081.1) but it may recognize isoform 2 lacking residue E81 (NP_001007470.1).
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name DAP10 / HCST (HCST Antibody Abstract)
Background Synonyms: HCST, DAP10, hematopoietic cell signal transducer, KAP10, PIK3AP, DNAX-activation protein 10, phosphoinositide-3-kinase adaptor protein
Gene ID 10870, 23900, 474146
NCBI Accession NP_001007470, NP_055081
Application Notes ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 40 kDa band observed in human spleen lysates (calculated MW of 9.5 kDa according to NP_055081.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wu et al, Science. 1999 Jul 30,285(5428):730-2., PMID: 10426994). Recommended concentration: 0.2-0.6 µg/mL. An additional band of unknown identity was also consistently observed at 100 kDa. This band was successfully blocked by incubation with the immunising peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-HCST antibody (Hematopoietic Cell Signal Transducer) (C-Term) (ABIN334384) ABIN334384 (0.2µg/ml) staining of human spleen lysate (35µg protein in RIPA buffer). ...
Background publications Upshaw, Arneson, Schoon, Dick, Billadeau, Leibson: "NKG2D-mediated signaling requires a DAP10-bound Grb2-Vav1 intermediate and phosphatidylinositol-3-kinase in human natural killer cells." in: Nature immunology, Vol. 7, Issue 5, pp. 524-32, 2006 (PubMed).