Hematopoietic Cell Signal Transducer (HCST) antibody

Antigen: Hematopoietic Cell Signal Transducer (HCST)

Synonyms: DAP10, KAP10, PIK3AP, DKFZP586C1522, Dap10, HCST

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334384

Additional Information

Alternative name: DAP10 / HCST

Gene ID: 10870, 23900 (mouse), 474146 (rat)

Immunogen: C-RSPAQEDGKVYIN

Description: All names and symbols: HCST, DAP10, hematopoietic cell signal transducer, KAP10, PIK3AP , DNAX-activation protein 10, phosphoinositide-3-kinase adaptor protein. Official Symbol: HCST. RefSeq number(s): NP_055081.1, NP_001007470.1

Specificity: This antibody is expected to recognise isoform 1 (NP_055081.1) but it may recognize isoform 2 lacking residue E81 (NP_001007470.1). Human, Mouse, Rat 93 aa, 92 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Approx 40kDa band observed in human spleen lysates (calculated MW of 9.5kDa according to NP_055081.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wu et al, Science. 1999 Jul 30,285(5428):730-2., PMID: 10426994). Recommended concentration: 0.2-0.6µg/ml. An additional band of unknown identity was also consistently observed at 100kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334384 (0.2µg/ml) staining of human spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Upshaw, Arneson, Schoon et al.: "NKG2D-mediated signaling requires a DAP10-bound Grb2-Vav1 intermediate and phosphatidylinositol-3-kinase in human natural killer cells." in: Nature immunology, Vol. 7, Issue 5, pp. 524-32, 2006 (PubMed).