NR1H4 antibody (Internal Region)

Catalog No. ABIN334396

Product Details anti-NR1H4 Antibody

Target: NR1H4 (Nuclear Receptor Subfamily 1, Group H, Member 4 (NR1H4))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NR1H4 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: Farnesoid X receptor / FXR

Sequence: KSCREKTELT PDQQ

Cross-Reactivity: Cow, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence KSCREKTELTPDQQ, from the internal region of the protein sequence according to NP_005114.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary testing showed a band at approx 55 kDa in Human Kidney, Colon, Duodenum and Ileum lysate after 0.5 μg/mL antibody staining (calculated MW of 54.4 kDa according to NP_005114.1). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NR1H4

Target: NR1H4 (Nuclear Receptor Subfamily 1, Group H, Member 4 (NR1H4))

Alternative Name: NR1H4 (NR1H4 Products)

Synonyms: zgc:110190 antibody, zgc:92742 antibody, NR1H4 antibody, BAR antibody, FXR antibody, HRR-1 antibody, HRR1 antibody, RIP14 antibody, AI957360 antibody, Fxr antibody, Rxrip14 antibody, nuclear receptor subfamily 1, group H, member 5 S homeolog antibody, nuclear receptor subfamily 1, group H, member 4 antibody, nuclear receptor subfamily 1 group H member 4 antibody, nr1h5.S antibody, nr1h4 antibody, NR1H4 antibody, Nr1h4 antibody

Background: Farnesoid X receptor, HRR1, NR1H4, nuclear receptor subfamily 1, group H, member 4 , BAR, FXR, HRR-1, RIP14 , farnesol receptor HRR-1

Gene ID: 9971

NCBI Accession: NP_005114

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Carbohydrate Metabolic Process