Glucagon Receptor (GCGR) antibody

Antigen: Glucagon Receptor (GCGR)

Synonyms: GCGR, GGR, FLJ97182, MGC138246

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN334409

Additional Information

Alternative name: Glucogon receptor

Gene ID: 2642

Immunogen: C-HGPPSKELQFGR

Description: All names and symbols: GCGR, glucagon receptor, GGR, MGC138246. Official Symbol: GCGR. RefSeq number(s): NP_000151.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 35kDa band in Human Kidney and Liver lysates after 0.03µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 54.0kDa according to NP_000151.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Sorensen, Winzell, Brand et al.: "Glucagon receptor knockout mice display increased insulin sensitivity and impaired beta-cell function." in: Diabetes, Vol. 55, Issue 12, pp. 3463-9, 2006 (PubMed).