Homeobox A5 (HOXA5) antibody
| Antigen |
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| Synonyms |
HOX1, HOX1C, HOX1.3, MGC9376, Hox-1.3, HOXA5, HOXA-5, hox1, hox1c, hox1.3, MGC138007 |
| Clonality |
Polyclonal |
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Host
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Application
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Western Blotting (WB) (31), Enzyme Immunoassay (EIA) (20), Flow Cytometry (FACS) (19), Immunofluorescence (IF) (18), ELISA (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Dot Blot (Dot) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
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| Catalog no. |
ABIN334419 |
| Quantity |
100 ug |
| Price |
369.20 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Homeobox A5
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Gene ID
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3202, 15402 (mouse)
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Immunogen
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C-DSHHGGKNSLSN
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Description
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All names and symbols: HOXA5, homeobox A5, HOX1, HOX1.3, HOX1C, MGC9376, homeo box 1C, homeo box A5, homeobox protein HOXA5. Official Symbol: HOXA5 . RefSeq number(s): NP_061975.2
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Specificity
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Human, Mouse
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave an approx 100kDa band in Human Breast and Heart lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 29.3kDa according to NP_061975.2. The 100kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Restrictions
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For Research Use only
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Publications
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Okada, Jiang, Lemieux et al.: "Leukaemic transformation by CALM-AF10 involves upregulation of Hoxa5 by hDOT1L." in: Nature cell biology, Vol. 8, Issue 9, pp. 1017-24, 2006 (PubMed).
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Alternatives for antigen "Homeobox A5 (HOXA5)", type "Antibodies"
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Hosts
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Rabbit (27), Mouse (23), Goat (2)
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Reactivities
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Human (48), Mouse (Murine) (21), Rat (Rattus) (20), Cow (Bovine) (3), Dog (Canine) (3), Horse (Equine) (3), Pig (Porcine) (3), Rabbit (3)
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Applications
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Western Blotting (WB) (31), Enzyme Immunoassay (EIA) (20), Flow Cytometry (FACS) (19), Immunofluorescence (IF) (18), ELISA (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Dot Blot (Dot) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term (1), C-Term E211 (1)
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Alternatives 
