KRIT1, Ankyrin Repeat Containing (KRIT1) antibody

Antigen: KRIT1, Ankyrin Repeat Containing (KRIT1)

Synonyms: CAM, CCM1, Ccm1, AA432855, AI450393, AI643869, BB155247, BB235701, 2010007K12Rik, A630036P20Rik, RGD1305929, krit1

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN334428

Additional Information

Alternative name: KRIT1 / CCM1

Gene ID: 889, 79264 (mouse)

Immunogen: C-YGNYESKKHKQG

Description: All names and symbols: KRIT1, KRIT1, ankyrin repeat containing , CAM, CCM1 , ankyrin repeat-containing protein Krit1, cerebral cavernous malformations 1, krev interaction trapped 1. Official Symbol: KRIT1. RefSeq number(s): NP_004903.2, NP_919436.1, NP_919437.1, NP_919438.1, NP_001013424.1

Specificity: This antibody is expected to recognise isoform 1 (NP_004903.2, NP_919436.1, NP_919437.1, NP_919438.1) and isoform 2 (NP_001013424.1). Human, Mouse, Rat, Dog 736 aa, 736 aa, 736 aa, 736 aa, 688 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments in lysates of several Human tissues and cell lines gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Zawistowski, Stalheim, Uhlik et al.: "CCM1 and CCM2 protein interactions in cell signaling: implications for cerebral cavernous malformations pathogenesis." in: Human molecular genetics, Vol. 14, Issue 17, pp. 2521-31, 2005 (PubMed).