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Monoglyceride Lipase (MGLL) (Internal Region), (N-Term) antibody

Details for Product No. ABIN334436, Supplier: Login to see
Antigen
  • cb909
  • wu:fc66g01
  • zgc:56561
  • MGC84195
  • MGLL
  • mgl
  • hu-k5
  • mgll
  • HU-K5
  • HUK5
  • MAGL
  • MGL
  • AA589436
  • Magl
  • Mgl
Epitope
N-Term, Internal Region
23
15
8
4
2
2
2
1
1
1
1
1
Reactivity
Human
121
24
24
3
2
1
1
1
1
Host
Goat
56
48
17
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
84
33
28
23
16
15
14
11
1
1
Supplier
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Immunogen Peptide with sequence C-QDLPHLVNADGQY, from the internal region (near N Terminus) of the protein sequence according to NP_009214.1, NP_001003794.1.
Blocking Peptide Blocking peptide for this product available: ABIN369607
Specificity This antibody is expected to recognize both reported isoforms (NP_009214.1, NP_001003794.1).
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Monoglyceride Lipase (MGLL Antibody Abstract)
Background Synonyms: monoglyceride lipase, HU-K5, MGLL, MGL, lysophospholipase-like
Gene ID 11343, 23945, 29254
NCBI Accession NP_001003794, NP_009214
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 30 kDa band observed in Human Adipose and Brain (Frontal Cortex) lysates (calculated MW of 33.3 kDa according to NP_001003794.1). In transfected HEK293 transiently expressing MGLL a band of approx. 36 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.5 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Monoglyceride Lipase (MGLL) (Internal Region), (N-Term) antibody (ABIN334436) ABIN334436 (0.5µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer)....
Background publications Blankman, Simon, Cravatt: "A comprehensive profile of brain enzymes that hydrolyze the endocannabinoid 2-arachidonoylglycerol." in: Chemistry & biology, Vol. 14, Issue 12, pp. 1347-56, 2007 (PubMed).