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MGLL antibody (Internal Region)

Verified MGLL Reactivity: Human, Mouse WB, IHC, ELISA, FACS, IF Host: Goat Polyclonal unconjugated
Catalog No. ABIN334436
  • Target See all MGLL Antibodies
    MGLL (Monoglyceride Lipase (MGLL))
    Binding Specificity
    • 8
    • 8
    • 8
    • 5
    • 4
    • 2
    • 1
    • 1
    Internal Region
    Reactivity
    • 39
    • 11
    • 7
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 40
    • 5
    • 1
    Goat
    Clonality
    • 42
    • 4
    Polyclonal
    Conjugate
    • 25
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MGLL antibody is un-conjugated
    Application
    • 31
    • 20
    • 10
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)
    Purpose
    Monoglyceride Lipase
    Sequence
    QDLPHLVNAD GQY
    Specificity
    This antibody is expected to recognize both reported isoforms (NP_009214.1, NP_001003794.1).
    Cross-Reactivity
    Cow, Dog, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-QDLPHLVNADGQY, from the internal region (near N Terminus) of the protein sequence according to NP_009214.1, NP_001003794.1.
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Liver, expression of MGLL is detected in hepatocytes. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 35 kDa band observed in Mouse Adipose lysates and in preliminary testing of Human, Cerebellum lysate and in lysate of cell line NIH3T3 (calculated MW of 33.3 kDa according to NP_001003794.1). Recommended concentration: 0.3-1 μg/mL. Pri

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and A431 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    MGLL (Monoglyceride Lipase (MGLL))
    Alternative Name
    MGLL (MGLL Products)
    Synonyms
    cb909 antibody, wu:fc66g01 antibody, zgc:56561 antibody, MGC84195 antibody, MGLL antibody, mgl antibody, hu-k5 antibody, mgll antibody, HU-K5 antibody, HUK5 antibody, MAGL antibody, MGL antibody, AA589436 antibody, Magl antibody, Mgl antibody, monoglyceride lipase antibody, monoglyceride lipase L homeolog antibody, 13L protein antibody, Monoglyceride lipase antibody, mgll antibody, MGLL antibody, mgll.L antibody, 13L antibody, YMTVg13L antibody, EHI_044210 antibody, EHI_045330 antibody, TATV_DAH68_039 antibody, Mgll antibody
    Background
    Monoglyceride lipase, HU-K5, MGLL, MGL, lysophospholipase-like
    Gene ID
    11343, 23945, 29254
    NCBI Accession
    NP_009214, NP_001003794
    Pathways
    Regulation of G-Protein Coupled Receptor Protein Signaling, Lipid Metabolism
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