Monoglyceride Lipase (MGLL) (N-Term), (Internal Region) antibody

Details for Product No. ABIN334436
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Antigen
Synonyms cb909, wu:fc66g01, zgc:56561, MGC84195, MGLL, mgl, hu-k5, mgll, HU-K5, HUK5, MAGL, MGL, AA589436, Magl, Mgl
Epitope
N-Term, Internal Region
(14), (7), (4), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(76), (16), (16), (14), (12), (12), (1)
Host
Goat
(40), (33), (5), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(54), (25), (21), (19), (14), (14), (10), (2), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN334436
374.27 $
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Immunogen Peptide with sequence C-QDLPHLVNADGQY, from the internal region (near N Terminus) of the protein sequence according to NP_009214.1, NP_001003794.1.
Specificity This antibody is expected to recognize both reported isoforms (NP_009214.1, NP_001003794.1).
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Monoglyceride Lipase
Background Synonyms: monoglyceride lipase, HU-K5, MGLL, MGL, lysophospholipase-like
Gene ID 11343, 23945, 29254
NCBI Accession NP_001003794, NP_009214
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 30 kDa band observed in Human Adipose and Brain (Frontal Cortex) lysates (calculated MW of 33.3 kDa according to NP_001003794.1). In transfected HEK293 transiently expressing MGLL a band of approx. 36 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.5 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Monoglyceride Lipase (MGLL) (N-Term), (Internal Region) antibody ABIN334436 (0.5µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Blankman, Simon, Cravatt: "A comprehensive profile of brain enzymes that hydrolyze the endocannabinoid 2-arachidonoylglycerol." in: Chemistry & biology, Vol. 14, Issue 12, pp. 1347-56, 2007 (PubMed).

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