NR4A1 antibody (Internal Region)

Catalog No. ABIN334451

Product Details anti-NR4A1 Antibody

Target: NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NR4A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: Nur77 / TR3 (isoform a)

Sequence: GRLPSKPKQP PDAS

Specificity: This antibody is expected to recognise isoform a (NP_002126.2 and NP_775180.1) only.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-GRLPSKPKQPPDAS, from the internal region of the protein sequence according to NP_002126.2, NP_775180.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Breast. Recommended concentration: 5 μg/mL.

Western Blot: Approx 60 kDa band observed in Human Thyroid gland lysates (calculated MW of 64.5 kDa according to NP_002126.2 and NP_775180.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NR4A1

Target: NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))

Alternative Name: NR4A1 (NR4A1 Products)

Synonyms: GFRP1 antibody, HMR antibody, N10 antibody, NAK-1 antibody, NGFIB antibody, NP10 antibody, NUR77 antibody, TR3 antibody, NGFI-B antibody, Gfrp antibody, Hbr-1 antibody, Hbr1 antibody, Hmr antibody, TIS1 antibody, nur77 antibody, Ngfi-b antibody, Nur77 antibody, nuclear receptor subfamily 4 group A member 1 antibody, nuclear receptor subfamily 4, group A, member 1 antibody, NR4A1 antibody, Nr4a1 antibody

Background: NR4A1, NUR77, nuclear receptor subfamily 4, group A, member 1 , GFRP1, HMR, MGC9485, N10, NAK-1, NGFIB, NP10, TR3 , TR3 orphan receptor, early response protein NAK1, growth factor-inducible nuclear protein N10, hormone receptor, orphan nuclear receptor HM

Gene ID: 3164, 15370, 79240

NCBI Accession: NP_002126, NP_775180

Pathways: Fc-epsilon Receptor Signaling Pathway, Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Steroid Hormone Mediated Signaling Pathway