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Patched 1 antibody (Internal Region)

Verified PTCH1 Reactivity: Zebrafish (Danio rerio) ELISA, IF Host: Goat Polyclonal unconjugated
Catalog No. ABIN334456
  • Target See all Patched 1 (PTCH1) Antibodies
    Patched 1 (PTCH1)
    Binding Specificity
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 38
    • 27
    • 11
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Zebrafish (Danio rerio)
    Host
    • 34
    • 5
    • 3
    Goat
    Clonality
    • 38
    • 4
    Polyclonal
    Conjugate
    • 30
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Patched 1 antibody is un-conjugated
    Application
    • 29
    • 21
    • 15
    • 9
    • 6
    • 5
    • 1
    • 1
    ELISA, Immunofluorescence (IF)
    Purpose
    Patched 1 (zebrafish)
    Sequence
    QTGSKKEPFN YSQ
    Cross-Reactivity
    Zebrafish (Danio rerio)
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-QTGSKKEPFNYSQ, from the internal region of the protein sequence according to NP_571063.1.
    Isotype
    IgG
    Top Product
    Discover our top product PTCH1 Primary Antibody
  • Application Notes
    Peptide ELISA: antibody detection limit dilution 1:32000.
    Comment

    Immunofluorescence: This product has been successfully used in IF studies of zebrafish tumors (Ju et al, Mol Cancer. 2009 Jun 25,8:40., PMID: 19555497). Recommended concentration: 1-3μg/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Ju, Spitsbergen, Eden, Taylor, Chen: "Co-activation of hedgehog and AKT pathways promote tumorigenesis in zebrafish." in: Molecular cancer, Vol. 8, pp. 40, (2009) (PubMed).

  • Target
    Patched 1 (PTCH1)
    Alternative Name
    ptc1 (PTCH1 Products)
    Synonyms
    BCNS antibody, HPE7 antibody, NBCCS antibody, PTC antibody, PTC1 antibody, PTCH antibody, PTCH11 antibody, lep antibody, ptc1 antibody, ptc2 antibody, Ptch antibody, Ptch2 antibody, Ptc-1 antibody, XPtc1 antibody, patched antibody, patched1 antibody, A230106A15Rik antibody, Ptc antibody, Ptc1 antibody, mes antibody, patched 1 antibody, patched 1 L homeolog antibody, Protein patched homolog 1 antibody, PTCH1 antibody, ptch1 antibody, Ptch1 antibody, ptch1.L antibody, ptc-1 antibody
    Background
    Ptc1, patched 1 , ptc-1, etID309849.2, patched1
    NCBI Accession
    NP_571063
    Pathways
    Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation
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