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PIK3CA antibody (Internal Region)

Verified PIK3CA Reactivity: Human IHC, ELISA Host: Goat Polyclonal unconjugated
Catalog No. ABIN334459
  • Target See all PIK3CA Antibodies
    PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))
    Binding Specificity
    • 15
    • 15
    • 13
    • 11
    • 9
    • 7
    • 7
    • 6
    • 6
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 84
    • 58
    • 32
    • 22
    • 15
    • 1
    Human
    Host
    • 88
    • 5
    • 1
    • 1
    Goat
    Clonality
    • 83
    • 12
    Polyclonal
    Conjugate
    • 29
    • 9
    • 8
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This PIK3CA antibody is un-conjugated
    Application
    • 75
    • 37
    • 29
    • 26
    • 26
    • 12
    • 6
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), ELISA
    Purpose
    PIK3CA / P110alpha
    Sequence
    HQWLKDKNKG EIYD
    Cross-Reactivity
    Cow, Dog, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-HQWLKDKNKGEIYD, from the internal region of the protein sequence according to NP_006209.2.
    Isotype
    IgG
    Top Product
    Discover our top product PIK3CA Primary Antibody
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Brain (Cerebral Cortex) shows cytoplasm stained in the cell bodies. Recommended concentration: 2-3 μg/mL. Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL. This antibody has been successfull

    Western Blot: Preliminary experiments in lysates of cell lines A431, HeLa and Jurkat gave no specific signal but low background (at antibody concentration up to 1 μg/mL).

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Abubaker, Bavi, Al-Haqawi, Jehan, Munkarah, Uddin, Al-Kuraya: "PIK3CA alterations in Middle Eastern ovarian cancers." in: Molecular cancer, Vol. 8, pp. 51, (2009) (PubMed).

  • Target
    PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))
    Alternative Name
    PIK3CA (PIK3CA Products)
    Synonyms
    CLOVE antibody, CWS5 antibody, MCAP antibody, MCM antibody, MCMTC antibody, PI3K antibody, p110-alpha antibody, C-P3K antibody, PI3-kinase antibody, PI3K-alpha antibody, pi3-k antibody, pi3k antibody, AA414921 antibody, C530050K14 antibody, p50alpha antibody, p55alpha antibody, p85alpha antibody, 6330412C24Rik antibody, caPI3K antibody, p110 antibody, p110alpha antibody, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha antibody, Phosphoinositide-3-kinase, catalytic, alpha polypeptide antibody, phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha antibody, phosphoinositide-3-kinase regulatory subunit 1 antibody, PIK3CA antibody, GL50803_14855 antibody, pik3ca antibody, Pik3ca antibody, Pik3r1 antibody
    Background
    PIK3CA, p110-alpha, phosphoinositide-3-kinase, catalytic, alpha polypeptide , MGC142161, MGC142163, PI3K, PI3-kinase p110 subunit alpha, PtdIns-3-kinase p110, phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha, phosphatidylinositol 3-kinase, catalyti
    Gene ID
    5290, 18706, 170911
    NCBI Accession
    NP_006209
    Pathways
    PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, VEGF Signaling
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