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Pituitary Homeobox 3 (PITX3) (Internal Region) antibody

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Antigen
Synonyms CTPP4, CTRCT11, PTX3, pitx3, Ptx3, ak, zgc:109716, pitx3-A, xPitx3
Epitope
Internal Region
(13), (13), (2), (1)
Reactivity
Human
(33), (17), (10), (5), (5), (2), (2), (1), (1), (1), (1), (1)
Host
Goat
(24), (15)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(33), (18), (9), (7), (6), (1), (1), (1)
Pubmed 1 reference available
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Quantity 100 μg
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Immunogen Peptide with sequence PEHGCKGQEHSD, from the internal region of the protein sequence according to NP_005020.1.
Blocking Peptide Blocking peptide for this product available: ABIN370277
Specificity This antibody is not excpected to cross-react with PITX1 and PITX2.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PITX3 (PITX3 Antibody Abstract)
Background Synonyms: PITX3, paired-like homeodomain 3, MGC12766, PTX3, homeobox protein PITX3
Gene ID 5309
NCBI Accession NP_005020
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 40 kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 31.8 kDa according to NP_005020.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Coulon et al, J Biol Chem. 2007 Nov 9,282(45):33192-200., PMID: 17848564). Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Image no. 1 for anti-Pituitary Homeobox 3 (PITX3) (Internal Region) antibody (ABIN334461) ABIN334461 (1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer)...
Background publications Kim, Inoue, Ishii et al.: "A MicroRNA feedback circuit in midbrain dopamine neurons." in: Science (New York, N.Y.), Vol. 317, Issue 5842, pp. 1220-4, 2007 (PubMed).

Catalog No. ABIN334461
$221.76
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