Pituitary Homeobox 3 (PITX3) (Internal Region) antibody

Details for Product No. ABIN334461
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Antigen
Synonyms CTPP4, CTRCT11, PTX3, pitx3, Ptx3, ak, zgc:109716, pitx3-A, xPitx3
Epitope
Internal Region
(8), (3), (1), (1)
Reactivity
Human
(25), (19), (16), (15), (15), (13), (12), (1), (1)
Host
Goat
(24), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(17), (10), (8), (4), (3), (3), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN334461
374.27 $
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Immunogen Peptide with sequence PEHGCKGQEHSD, from the internal region of the protein sequence according to NP_005020.1.
Specificity This antibody is not excpected to cross-react with PITX1 and PITX2.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PITX3
Background Synonyms: PITX3, paired-like homeodomain 3, MGC12766, PTX3, homeobox protein PITX3
Gene ID 5309
NCBI Accession NP_005020
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx 40 kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 31.8 kDa according to NP_005020.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Coulon et al, J Biol Chem. 2007 Nov 9,282(45):33192-200., PMID: 17848564). Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Pituitary Homeobox 3 (PITX3) (Internal Region) antibody ABIN334461 (1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Kim, Inoue, Ishii et al.: "A MicroRNA feedback circuit in midbrain dopamine neurons." in: Science (New York, N.Y.), Vol. 317, Issue 5842, pp. 1220-4, 2007 (PubMed).

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