Prostaglandin E Receptor 3 (Subtype EP3) (PTGER3) antibody

Antigen: Prostaglandin E Receptor 3 (Subtype EP3) (PTGER3)

Synonyms: EP3, EP3e, EP3-I, EP3-II, EP3-IV, EP3-III, MGC27302, MGC141828, MGC141829, PTGER3

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN334464

Additional Information

Alternative name: Prostaglandin E Receptor 3

Gene ID: 5733

Immunogen: HCKTHTEKQKECN

Description: All names and symbols: PTGER3, prostaglandin E receptor 3 (subtype EP3) , EP3, EP3-I, EP3-II, EP3-III, EP3-IV, EP3e, MGC27302 , PGE receptor, EP3 subtype, alternative splicing, prostaglandin E receptor 3, subtype EP3, prostaglandin E2 receptor, prostaglandin receptor (PGE-2), prostanoid EP3 receptor. Official Symbol: PTGER3. RefSeq number(s): NP_000948.2, NP_942005.1, NP_942006.1, NP_942007.1, NP_942012.1, NP_942013.1, NP_942008.1, NP_942009.1, NP_942010.1, NP_942011.1

Specificity: This antibody is expected to recognise isoform 1 (NP_000948.2), isoform 2 (NP_942005.1), isoform 3 (NP_942006.1), isoform 4 (NP_942007.1, NP_942012.1 and NP_942013.1), isoform 5 (NP_942008.1), isoform 6 (NP_942009.1), isoform 7 (NP_942010.1) and isoform 8 (NP_942011.1). Human, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:1,000. Western Blot: Preliminary experiments gave an approx 25kDa band in human kidney, lung, placenta, and lymph node lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated sizes varying from 47.3kDa according to NP_000948.2 down to 40.5kDa according to NP_942010.1. The 25kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Gerlo, Verdood, Gellersen et al.: "Mechanism of prostaglandin (PG)E2-induced prolactin expression in human T cells: cooperation of two PGE2 receptor subtypes, E-prostanoid (EP) 3 and EP4, via calcium- and cyclic adenosine 5'-monophosphate-mediated signaling pathways." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 10, pp. 5952-62, 2004 (PubMed).