Prostaglandin E Receptor 3 (Subtype EP3) (PTGER3) antibody

Antigen: Prostaglandin E Receptor 3 (Subtype EP3) (PTGER3)

Synonyms: EP3, EP3e, EP3-I, EP3-II, EP3-IV, EP3-III, MGC27302, MGC141828, MGC141829, PTGER3

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334469

Additional Information

Alternative name: PTGER3 / EP3

Gene ID: 5733

Immunogen: KLLREPCSVQLS

Description: All names and symbols: PTGER3, EP3, prostaglandin E receptor 3 (subtype EP3) , EP3-I, EP3-II, EP3-III, EP3-IV, EP3e, MGC141828, MGC141829, MGC27302, PGE receptor, EP3 subtype, prostaglandin E receptor 3, subtype EP3, prostaglandin E2 receptor, prostaglandin receptor (PGE-2), pr. Official Symbol: PTGER3. RefSeq number(s): NP_000948.2, NP_942005.2, NP_942006.1

Specificity: This antibody is expected to recognize isoforms 1, 2 and 3 (NP_000948.2, NP_942005.2 and NP_942006.1). Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:2,000. Western Blot: Approx 55kDa band observed in Human Adipose, Kidney, Pancreas and Placenta lysates (calculated MW of 47.3kDa according to NP_000948.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sung et al, , Cancer Res. 2005 Oct 15,65(20):9304-11, PMID: 16230392). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334469 (1µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Sarrazin, Hackett, Fortier et al.: "Role of EP3 and EP4 prostaglandin receptors in reorganization of the cytoskeleton in mature human osteoclasts." in: The Journal of rheumatology, Vol. 31, Issue 8, pp. 1598-606, 2004 (PubMed).