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SPI1 antibody (Internal Region)

Verified SPI1 Reactivity: Human WB, ELISA, IHC Host: Goat Polyclonal unconjugated
Catalog No. ABIN334471
  • Target See all SPI1 Antibodies
    SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
    Binding Specificity
    • 15
    • 11
    • 9
    • 7
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 86
    • 26
    • 19
    • 7
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    Human
    Host
    • 82
    • 7
    • 1
    Goat
    Clonality
    • 72
    • 19
    Polyclonal
    Conjugate
    • 48
    • 6
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This SPI1 antibody is un-conjugated
    Application
    • 69
    • 32
    • 24
    • 15
    • 13
    • 13
    • 12
    • 9
    • 6
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Purpose
    PU.1
    Sequence
    DLYQRQTHEY Y
    Specificity
    This antibody is expected to recognise both reported isoforms (NP_001074016.1, NP_003111.2).
    Cross-Reactivity
    Human, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-DLYQRQTHEYY, from the internal region of the protein sequence according to NP_001074016.1, NP_003111.2.
    Isotype
    IgG
    Top Product
    Discover our top product SPI1 Primary Antibody
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Spleen and Colon (lymphoid aggregate). Recommended concentration: 3.75 μg/mL.

    Western Blot: Approx 40 kDa band observed in lysates of DAUDI and K562 (calculated MW of 31.2 kDa according to NP_001074016.1 and of 31.1 kDa according to NP_003111.2). The observed molecular weight corresponds to earlier findings in literature with differe

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
    Alternative Name
    SPI1 (SPI1 Products)
    Synonyms
    OF antibody, PU.1 antibody, SFPI1 antibody, SPI-1 antibody, SPI-A antibody, Dis-1 antibody, Dis1 antibody, Sfpi-1 antibody, Sfpi1 antibody, Spi-1 antibody, Tcfpu1 antibody, Tfpu.1 antibody, Pu.1 antibody, cb1086 antibody, pu.1 antibody, pu1 antibody, sfpi1 antibody, si:by184l24.2 antibody, spi1 antibody, SPI1/PU.1 antibody, spi1l antibody, zgc:152991 antibody, Spi-1 proto-oncogene antibody, spleen focus forming virus (SFFV) proviral integration oncogene antibody, Spi-1 proto-oncogene b antibody, Spi-1 proto-oncogene a antibody, SPI1 antibody, Spi1 antibody, spi1b antibody, spi1a antibody
    Background
    PU.1, SPI1, spleen focus forming virus (SFFV) proviral integration oncogene spi1 , hCG_25181, SFPI1, SPI-1, SPI-A, 31 kDa transforming protein, SPI-1 proto-oncogene, hematopoietic transcription factor PU.1
    Gene ID
    6688, 366126
    NCBI Accession
    NP_001074016, NP_003111
    Pathways
    Stem Cell Maintenance
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