Drosha, Ribonuclease Type III (DROSHA) antibody

Antigen: Drosha, Ribonuclease Type III (DROSHA)

Synonyms: Rn3, Drosha, Etohi2, AI874853, MGC115770, 1110013A17Rik, RN3, DROSHA, ETOHI2, RANSE3L, RNASE3L, HSA242976, im:7150667, zgc:158612, RIBONUCLEASE 3, RNS3, T24P13.23

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334479

Additional Information

Alternative name: RNASEN / Drosha

Gene ID: 29102, 14000 (mouse), 310159 (rat)

Immunogen: C-KQTDKQKLAQRE

Description: All names and symbols: Rnasen, Drosha, ribonuclease III, nuclear , 1110013A17Rik, AI874853, Etohi2, MGC115770, Rn3 , ethanol induced 2, ribonuclease III. Official Symbol: RNASEN. RefSeq number(s): NP_081075.2

Specificity: This antibody also cross-reacts with human Ribonuclease III (GeneID 29102, NP_037367.2). Human, Mouse, Rat, Dog 1303 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 150kDa band observed in Human Liver lysates (calculated MW of 151kDa according to NP_081075.2 and 159kDa according to Human NP_037367.2). Recommended concentration: 0.5-1.5µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334479 (0.5µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Thomson, Newman, Parker et al.: "Extensive post-transcriptional regulation of microRNAs and its implications for cancer." in: Genes & development, Vol. 20, Issue 16, pp. 2202-7, 2006 (PubMed).