Roundabout, Axon Guidance Receptor, Homolog 2 (Drosophila) (ROBO2) antibody

Antigen: Roundabout, Axon Guidance Receptor, Homolog 2 (Drosophila) (ROBO2)

Synonyms: SAX3, KIAA1568, ROBO2

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN334482

Additional Information

Alternative name: ROBO2

Gene ID: 6092, 268902 (mouse), 84409 (rat)

Immunogen: C-NQSQRPRPTKKHK

Description: All names and symbols: ROBO2, roundabout, axon guidance receptor, homolog 2 (Drosophila), KIAA1568, SAX3, roundabout, axon guidance receptor, homolog 2. Official Symbol: ROBO2. RefSeq number(s): NP_002933.1

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave an approx. 75kDa band in Human Brain (Amygdala) lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 151kDa according to NP_002933.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Fujisawa, Wrana, Culotti: "The slit receptor EVA-1 coactivates a SAX-3/Robo mediated guidance signal in C. elegans." in: Science (New York, N.Y.), Vol. 317, Issue 5846, pp. 1934-8, 2007 (PubMed).