Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E) (internal) antibody

Antigen: Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E)

Synonyms: SemD, SEMA1, SEMAD, SEMAL, coll-1, Hsema-I, SEMAIII, Hsema-III, MGC133243, SEMA3E, fj37c11, wu:fj37c11, si:ch211-120m13.2

Epitope: internal

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN334487

Additional Information

Alternative name: Semaphorin 3E (internal)

Gene ID: 9723, 20349 (mouse)

Immunogen: C-NGGRYGTTKDYPDD

Description: All names and symbols: SEMA3E, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E, KIAA0331, M-SEMAH, M-SemaK, M-sema H, SEMAH, coll-5 , sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3E, semaphorin 3E. Official Symbol: SEMA3E. RefSeq number(s): NP_036563.1

Specificity: Human, Mouse, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Preliminary experiments gave an approx 35kDa band in Human Prostate lysates after 0.03µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 89.2kDa according to NP_036563.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Gu, Yoshida, Livet et al.: "Semaphorin 3E and plexin-D1 control vascular pattern independently of neuropilins." in: Science (New York, N.Y.), Vol. 307, Issue 5707, pp. 265-8, 2005 (PubMed).