SMO (C-Term) antibody

Antigen: SMO

Binding Site: C-Term

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334494

Additional Information

Gene ID: 6608

Immunogen: C-NLMDTELMDADSD

Description: All names and symbols: SMO, smoothened homolog (Drosophila) , HGNC:11119, Gx, SMOH , seven transmembrane helix receptor, smoothened. Official Symbol: SMO. RefSeq number(s): NP_005622.1

Specificity: Human, Dog, Zebrafish 787 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Approx 60kDa band observed in Human Bone Marrow lysates (calculated MW of 86.4kDa according to NP_005622.1). The observed molecular weight corresponds to findings with ABIN334495, a product of different design reacting to a different part of the same protein. Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

A) EB07026 and B) ABIN334495 (0.3µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Corbit, Aanstad, Singla et al.: "Vertebrate Smoothened functions at the primary cilium." in: Nature, Vol. 437, Issue 7061, pp. 1018-21, 2005 (PubMed).