SMO (C-Term) antibody

Details for Product No. ABIN334494
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Antigen
Synonyms FZD11, Gx, SMOH, Smoh, smoh, CHUNP6862, wu:fb70b01, wu:fc39h03, bnb, smoothened, E130215L21Rik
Epitope
C-Term
(8), (6), (6), (3), (2), (1), (1), (1)
Reactivity
Human
(44), (16), (15), (4), (3), (1), (1)
Host
Goat
(20), (18), (7)
Clonality
Polyclonal
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(35), (31), (5)
Pubmed 1 reference available
Catalog no. ABIN334494
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence C-NLMDTELMDADSD, from the C Terminus of the protein sequence according to NP_005622.1.
Cross-Reactivity Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Background Synonyms: SMO, smoothened homolog (Drosophila), HGNC:11119, Gx, SMOH, seven transmembrane helix receptor, smoothened
Gene ID 6608
NCBI Accession NP_005622.1
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60 kDa band observed in Human Bone Marrow lysates (calculated MW of 86.4 kDa according to NP_005622.1). The observed molecular weight corresponds to findings with EB07444, a product of different design reacting to a different part of the same protein. Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-SMO (C-Term) antibody A) EB07026 and B) ABIN334495 (0.3µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Corbit, Aanstad, Singla et al.: "Vertebrate Smoothened functions at the primary cilium." in: Nature, Vol. 437, Issue 7061, pp. 1018-21, 2005 (PubMed).

Hosts (20), (18), (7)
Reactivities (44), (16), (15), (4), (3), (1), (1)
Applications (35), (31), (5)
Epitopes (8), (6), (6), (3), (2), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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