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SMO (C-Term) antibody

Details for Product No. ABIN334494, Supplier: Log in to see
Antigen
  • CHUNP6862
  • smoh
  • wu:fb70b01
  • wu:fc39h03
  • E130215L21Rik
  • Smoh
  • bnb
  • smoothened
  • FZD11
  • Gx
  • SMOH
  • B130066H01Rik
  • PAO
  • PAOh1
  • SMO
Epitope
C-Term
21
13
13
11
9
8
8
7
5
4
3
1
1
1
Reactivity
Human
94
25
25
6
4
4
2
2
2
2
Host
Goat
47
27
22
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
65
57
11
7
6
5
1
Supplier
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Immunogen Peptide with sequence C-NLMDTELMDADSD, from the C Terminus of the protein sequence according to NP_005622.1.
Blocking Peptide Blocking peptide for this product available: ABIN369484
Cross-Reactivity Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Background Synonyms: SMO, smoothened homolog (Drosophila), HGNC:11119, Gx, SMOH, seven transmembrane helix receptor, smoothened
Gene ID 6608
NCBI Accession NP_005622
Pathways Hedgehog Signaling
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60 kDa band observed in Human Bone Marrow lysates (calculated MW of 86.4 kDa according to NP_005622.1). The observed molecular weight corresponds to findings with EB07444, a product of different design reacting to a different part of the same protein. Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-SMO (C-Term) antibody (ABIN334494) A) EB07026 and B) ABIN334495 (0.3µg/ml) staining of Human Bone Marrow lysate (35µg pr...
Background publications Corbit, Aanstad, Singla et al.: "Vertebrate Smoothened functions at the primary cilium." in: Nature, Vol. 437, Issue 7061, pp. 1018-21, 2005 (PubMed).