Smoothened Homolog (Drosophila) (SMO) (internal) antibody

Antigen: Smoothened Homolog (Drosophila) (SMO)

Synonyms: Gx, SMOH, Smoh, smoh, CHUNP6862, wu:fb70b01, wu:fc39h03, bnb, smoothened, E130215L21Rik, XSmo

Epitope: internal

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334495

Additional Information

Alternative name: SMO (internal)

Gene ID: 6608, 319757 (mouse), 25273 (rat)

Immunogen: C-QSDDEPKRIKKS

Description: All names and symbols: SMO, smoothened homolog (Drosophila) , Gx, SMOH , seven transmembrane helix receptor, smoothened . Official Symbol: SMO. RefSeq number(s): NP_005622.1

Specificity: Human, Mouse, Rat, Dog, Zebrafish 787 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:1,000. Approx 60kDa band observed in Human Bone Marrow lysates (calculated MW of 86.4kDa according to NP_005622.1). The observed molecular weight corresponds to findings with ABIN334494, a product of different design reacting to a different part of the same protein. Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

A) EB07026 and B) ABIN334495 (0.3µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Taipale, Cooper, Maiti et al.: "Patched acts catalytically to suppress the activity of Smoothened." in: Nature, Vol. 418, Issue 6900, pp. 892-7, 2002 (PubMed).