Sorting Nexin 17 (SNX17) antibody

Antigen: Sorting Nexin 17 (SNX17)

Synonyms: KIAA0064, AI790646, mKIAA0064, D5Ertd260e, 5830447M19Rik, snx17, MGC53167, MGC69341, MGC198061, MGC83142, SNX17, MGC139637, wu:fb67g03, wu:fb67g09, wu:fc27g08, wu:fj81d04, si:ch211-101l18.2, DKFZp459P1011

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN334496

Additional Information

Alternative name: SNX17

Gene ID: 9784, 266781 (mouse), 298836 (rat)

Immunogen: C-RKSYWDSAYDD

Description: All names and symbols: SNX17, sorting nexin 17 , KIAA0064. Official Symbol: SNX17. RefSeq number(s): NP_055563.1

Specificity: Human, Mouse, Rat, Dog 470 aa

Synonyms: KIAA0064, AI790646, mKIAA0064, D5Ertd260e, 5830447M19Rik, snx17, MGC53167, MGC69341, MGC198061, MGC83142, SNX17, MGC139637, wu:fb67g03, wu:fb67g09, wu:fc27g08, wu:fj81d04, si:ch211-101l18.2, DKFZp459P1011

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Preliminary experiments gave an approx 37kDa band in lysates of cell lines A431, A549, HEK293, HepG2, after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 52.9kDa according to NP_055563.1. The 37kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Transporters

Restrictions: For Research Use only

Publications

Publications: Czubayko, Knauth, Schlueter et al.: "Sorting nexin 17, a non-self-assembling and a PtdIns(3)P high class affinity protein, interacts with the cerebral cavernous malformation related protein KRIT1." in: Biochemical and biophysical research communications, Vol. 345, Issue 3, pp. 1264-72, 2006 (PubMed).