Trace Amine Associated Receptor 1 (TAAR1) antibody

Antigen: Trace Amine Associated Receptor 1 (TAAR1)

Synonyms: Tar1, Trar1, TA1, TAR1, TRAR1, MGC126874, MGC138399, RP11-295F4.9

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN334501

Additional Information

Alternative name: Taar1 / Tar1(mouse)

Gene ID: 111174 (mouse), 113914 (rat)

Immunogen: C-EGKSQAPQSKETK

Description: All names and symbols: Taar1, trace amine associated receptor 1, TA1, TAR1, TRAR1, trace amine receptor 1, trace-amine associated receptor 1. Official Symbol: Taar1. RefSeq number(s): NP_444435.1

Specificity: Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave bands at approx 38kDa, but also at approx 90kDa and 75kDa in lysates of Mouse Brain and of cell line K562 after 0.03 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the extra bands we observe given the calculated size of 39.1kDa according to NP_444435.1. All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Reese, Bunzow, Arttamangkul et al.: "Trace amine-associated receptor 1 displays species-dependent stereoselectivity for isomers of methamphetamine, amphetamine, and para-hydroxyamphetamine." in: The Journal of pharmacology and experimental therapeutics, Vol. 321, Issue 1, pp. 178-86, 2007 (PubMed).