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TAR DNA Binding Protein (TARDBP) (Internal Region) antibody

Details for Product No. ABIN334503, Supplier: Log in to see
Antigen
  • 1190002A23Rik
  • ALS10
  • C85084
  • DKFZp459I2127
  • TARDBP
  • tardbp
  • TDP-43
  • TDP43
  • Tdp43
  • wu:fb77f02
  • wu:fc52g10
Epitope
Internal Region
28
26
19
16
15
13
13
10
8
7
6
4
3
3
2
2
1
1
Reactivity
Human, Mouse (Murine)
225
103
80
28
23
19
16
15
8
8
6
4
2
1
Host
Goat
124
93
14
1
Clonality
Polyclonal
Conjugate
Un-conjugated
10
9
7
6
5
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
212
110
69
61
49
47
19
11
10
5
5
4
3
2
Supplier
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Immunogen Peptide with sequence C-HISNAEPKHNSNRQ, from the internal region of the protein sequence according to NP_031401.1.
Blocking Peptide Blocking peptide for this product available: ABIN370306
Cross-Reactivity Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name TARDBP (TARDBP Antibody Abstract)
Background Synonyms: TARDBP, TAR DNA binding protein, ALS10, TDP-43, OTTHUMP00000002171, TAR DNA-binding protein-43
Gene ID 23435, 230908
NCBI Accession NP_031401
Research Area Chromatin and Nuclear Signaling, Transcription Factors, DNA/RNA, Hormones, Cytokines
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 45 kDa band observed in lysates of cell lines Jurkat and NIH3T3 (calculated MW of 44.7 kDa according to NP_031401.1). A slightly larger protein is detected in some regions of the brain. Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-TAR DNA Binding Protein (TARDBP) (Internal Region) antibody (ABIN334503) ABIN334503 (0.3 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Prim...
Background publications Zhang, Xu, Dickey, Buratti, Baralle, Bailey, Pickering-Brown, Dickson, Petrucelli: "Progranulin mediates caspase-dependent cleavage of TAR DNA binding protein-43." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 39, pp. 10530-4, 2007